2019•Unpublished venueRequires access
The effection of transplantation of bone marrow mesenchymal stem cells on cardiac function of rats of dilated cardiomyopathy with heart failure
Qianxiao Li, Qin Yu, Rongmei Na
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Abstract
Objective
To explore the effects on cardiac function of rats of dilated cardiomyopathy (DCM) with heart failure by peripheral vein injection of bone marrow mesenchymal stem cells (MSCs).
Methods
The animal models of DCM with heart failure (n=64) were established by the classic method of intraperitoneal injection doxorubicin (2.5 mg/kg, once per week, for 6 weeks, the total volume was 15 mg/kg). Then, they were divided into MSCs transplantation group (n=32) and control group (n=32). The rats of MSCs transplantation group received injection of cell suspension with MSCs via the tail vein. Cell number was 5×106 each rat and the injection volume was 0.5 ml each time, a total of 10 times, once every other day. The rats of control group received injection of serum-free medium excluding MSCs and the injection volume and method was the same as MSCs transplantation group. All of the rats were fed under the same conditions and underwent echocardiography for left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) in the 10th week after MSCs transplantation respectively.
Results
Cardiac ultrasound examination showed that LVEDD and LVESD of MSCs transplantation group ((6.1±0.3) mm, (3.4±0.2) mm) were significantly lower than control group ((7.6±0.2) mm, (5.3±0.3) mm) and the difference was statistically significant (P<0.05). LVEF and LVFS of MSCs transplantation group ((79.8±0.9)%, (42.5±0.6)%) were significantly higher than control group ((64.1±0.8)%, (30.2±0.7)%) and the difference was statistically significant (P<0.05).
Conclusion
For doxorubicin-induced rats of DCM with heart failure, their cardiac function can be improved to some extent by peripheral vein injection of MSCs.
Key words:
Bone marrow mesenchymal stem cells transplantation; Dilated cardiomyopathy; Cardiac function