Observation of effect of transplantation of bone marrow mesenchymal stem cells on rabbit cardiac function after myocardial infarction by echocardiography

Objective To investigate the roles of transplantation of bone marrow-derived mesenchymal stem cells(MSCs) on rabbit post-infarct myocardium and their influences on cardiac function.Methods The myocardial infarction model was made by using coronary artery ligation of the left anterior descending.After 2 weeks,the self pulp MSCs,5-aza induced MSCs,and bone marrow mononuclear cells(BMMNCs) labeled with Dil were injected into ischemic myocardium of rabbits,and the injection of serum-free media used as the control group.Two-dimensional ultrasound measurements of left ventricular end-systolic diameter(LVESD),left ventricular end-diastolic diameter(LVEDD),left ventricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS) index evaluation cardiac function were carried out before operation,before and after transplantation of 2 weeks and 4 weeks.Results After implantation of 2 and 4 weeks,the myocardial function (LVEF and LVFS value) of rabbits implanted with MSCs,5-aza induced MSCs was better than control group(P 0.05).BMMNCs group improved after transplantation of 4 weeks(P 0.05).No significant difference of LVESD,LVEDD was found between cells transplantation groups and control group(P 0.05).Conclusion MSCs,5-aza induced MSCs,BMMNCs were lived in myocardial infarction,and improved left ventricular systolic function after myocardial ischemia.