Study of the feasibility of bone marrow MSCs injected through systemic vein to improve heart function after myocardial infarction

Objective:To study the feasibility of intraveinous injected MSCs to delay heart function deterioration,and distribution of the transplanted cells in organs.Methods:Bone marrow MSCs were injected into the subglossal vein of the SD rats on 7th day after the myocardial infarction were created,and the organs such as lung,heart,liver,renal,spleen were harvested at different time after transplantation.In order to assess the efficiency of this approach of cell transplantation,24 SD rats were averagely divided into experiment group and control group,MSCs or medium was given.The diameters and function were evaluated through transthoracic echocardiography after 3 weeks and 6 weeks.Results:After injection,the MSCs could distributed into the heart and non-targeted organ,such as spleen,renal,lung,but scarcely in the liver,the structure of these organs remain normal.The cells migrating into myocardium had a tendency to the infracted area.The left ventricle function did not exacerbate after injection of MSCs compared with control group[MSCs∶control:LVESd(0.92±0.16)∶(1.078± 0.15)cm;LVEDd(0.66±0.13)∶(0.79±0.11)cm;FS(28.4±4.2)∶(24.3±3.1)%;LVEF(52.7±4)∶(42.89±4.2)%,P0.05 all].Conclusion:The intravenously injection of MSCs is a feasible and safe approach to restore the heart function after MI.