卜丽莎, 高申, 华坤, 李洪岩, 李广生
Abstract
目的观察不同浓度氟对体外培养的小鼠成骨细胞增殖活力的影响.方法采用胶原酶消化分离培养小鼠乳鼠颅盖骨成骨细胞,应用四唑盐(MTT)比色法,观察氟对培养的成骨细胞增殖活力的影响.结果染氟0.5 mg/L以下,细胞增殖活力变化不明显,染氟1.0～4.0 mg/L时成骨细胞增殖活力明显增强,与对照组比较差异有显著意义,且此作用呈剂量和时间依赖性.结论氟在一定剂量范围对体外培养的小鼠成骨细胞的增殖有促进作用.
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目的观察不同浓度氟对体外培养的小鼠成骨细胞增殖活力的影响.方法采用胶原酶消化分离培养小鼠乳鼠颅盖骨成骨细胞,应用四唑盐(MTT)比色法,观察氟对培养的成骨细胞增殖活力的影响.结果染氟0.5 mg/L以下,细胞增殖活力变化不明显,染氟1.0～4.0 mg/L时成骨细胞增殖活力明显增强,与对照组比较差异有显著意义,且此作用呈剂量和时间依赖性.结论氟在一定剂量范围对体外培养的小鼠成骨细胞的增殖有促进作用.
Key concepts: In vitro, Osteoblast, Cell biology, Chemistry, Biology, Biochemistry