Influence of fluoride on proliferation activity of cultivated osteob lasts of mouse in vitro

ObjectiveTo study the effects of fluoride on proliferation activity of cultivated osteoblasts of mouse in vitro.MethodsOsteoblasts were digested by collagenase from n eonatal mouse and cultured. MTT colorimetry was used to detect osteoblastic prol iferation activity.ResultsThe fluoride below 0.5 mg/L didn’t influence cell proliferation activity, but 1.0～4.0 mg/L of fluoride could increase signif i cantly osteoblastic proliferation activity, and the fluoride effects were dose a nd time-dependent.ConclusionsFluoride in certain range of cententration can elevate osteoblastic proliferation activity.