Application of glycerol blood chocolate decoction in the preservation of Haemophilus influenzae

Objective To evaluate the application value of glycerol blood chocolate soup in the bacteria preservation of Haemophilus influenzae. Methods Haemophilus influenzae strains ATCC10211, ATCC49247 and ATCC49766 were saved into glycerol blood chocolate soup, then it was stored in refrigerator at below -35℃.The preserved strains from the refrigerator at 1 month, 3 months, 6 months, 1 year, 2 years were taken out, and dissolved it at 35℃ for 5min, and then immediately transferred it to chocolate agar plate, placed it in the 35℃, CO2 environment overnight.Observed the survival of strains, colony morphology and whether the demand factor test had change.Saved the standard strains ATCC49247 and ATCC49766 2 years for the paper dispersion method drug sensitive test. Results After 2 years of observation, 3 strains of Haemophilus influenzae strains grew well in the survival rate of 100%, the colony was colorless, transparent and flat moist, it could be observed gram small negative bacilli by Gram staining, blood agar satellite test was positive, no hemolysis, Columbia satellite test was negative.All traits were the characteristics of Haemophilus influenzae.The drug sensitivity characteristics of ATCC49247, ATCC49766 standard strains were still in line with the requirements of CLSI. Conclusion Glycerol blood chocolate soup is suit for Haemophilus influenzae bacteria preservation. Key words: Haemophilus; Preservation, Biological; Culture Media