The relationship between miR-195 expression and clinical pathological characteristics of colorectal cancer

Objective To explore the relationship between miR-195 expression and clinical pathological characteristics of colorectal cancer.Methods The expression of miR-195 in 50 cases of colorectal cancer tissue and adjacent non-tumor mucosa (5 cm away from the cancer tissue) was detected by real time PCR.The relationship between miR-195 expression and clinical pathological characteristics of colorectal cancer was analyzed.Results miR-195 CpG island methylate phenotype was positive in 58％ (29/50) of patients with colorectal cancer.The expression level of miR-195 in 34 patients colorectal cancer tissue was lower than that in adjacent non-tumor mucosa,that was T/N ＜ 2.The expression level of miR-195 in carcinoma tissue was 0.837 ± 1.145,comparing with adjacent non-tumor mucosa (2.236 ± 2.468),showing significant difference (P ＜ 0.05).Down-regulated expression of miR-195 in carcinoma tissue was independent of age,gender,site(P ＞ 0.05),and related to lymphnode metastasis and TNM stage(P ＜ 0.05).Patients with lymph node metastasis and advanced TNM stage showed significantly reduced expression of miR-195.Conclusions The tendency of common low expression level of miR-195 in human colorectal cancer.MiR-195 maybe play as suppressor gene in human colon carcinoma.Hypermethylation of miR-195 promoter may be one of reasons leading to down-regulation of miR-195 in colorectal cancer tissues and participated in the occurrence and development of human colon carcinoma. Key words: Colorectal neoplasms;  Methylation