The research of spleen deftciency rats'monoamine neurotransmitter and the treatment mechanism of Sijunzi Decoction

Objective To analyze the change of spleen deficiency rats' monoamine neurotransmitter and the treatment mechanism of Sijunzi decoction. Methods 60 rats were divided into control group, spleen deficiency group and Sijunzi Decoction group. And every group had 20 rats. Then high performance liquid chromatograph was used to analyze rats'5-HT,DA,5-HIAA,DOPAC and HVA, in their corpus striatum. Results Compared with control group,the concentration of 5-HT[(2.25±0.78)ng/mg]in spleen deficiency rats significantly ascended, and DA[(26.06±9.01)ng/mg]significantly descended(P＜0.01).After the treatment of Sijunzi decoction, the level of 5-HT[(1.86±0.53)ng/mg]and DA[(38.95±10.86)ng/mg]came back to normal. And compared with spleen deficiency rats, treatment rats' DA significantly ascended(P＜0.05).Conclusion There is a consanguineous relationship between spleen deficiency syndrome and rats' monoamine neurotransmitter,and spleen deficiency syndrome has a central mechanism.And Sijunzi decoction could be used to treat with spleen deficiency syndrome by regulating monoamine neurotransmitter. Key words: Spleen deficiency syndrome ;  Monoamine neurotransmitter ;  Sijunzi decoction ;