白细胞介素-1β,-1Rα对胚胎-内膜共培养体系分泌基质金属蛋白酶-9/细胞间黏附分子-1的影响

Objective To research the effects of interleukin(IL)-1β and IL-1Rα on the embryo and endometrium co-culture system. Methods A total of 30 female Kunming white mice (6 to 8 weeks) and 15 male mice (12 to 16 weeks) were selected. 8 cell stage embryos and endometrial glandular epithelial cells were collected and the endometrial cells were primarily cultured. There were 7 copies primary endometrial cells were successful cultured and were inoculated into 35 holes respectively. Control group were 5 hole cells without any treatment (n=5×7). 30 holes was divided into 6 groups according to different concentrations of IL-1β and different raio of IL-1β+ IL-1Rα. 3 groups were GⅡ with 1 ng/mL IL-1β, 10 ng/mL IL-1β and 100 ng/mL IL-1β, 3 groups were GⅡ with 1 ng/mL IL-1β+ 10 μg/mL IL-1Rα, 10 ng/mL IL-1β+ 10 μg/mL IL-1Rα and 100 ng/mL IL-1β+ 10 μg/mL IL-1Rα (n=5×7). 8 cell embryos were co-cultured with endometrial glandular epithelial cells in different media (6 embryos each hole). Supernatant were collected after 48 h co-culture, and the levels of matrix metalloproteinase-9 (MMP-9) / intercellular adhesion molecule-1 (ICAM-1) were measured by ELISA. Results Expressions of MMP-9 / ICAM-1 were significantly increased in 1 ng/mL IL-1β group and 10 ng/mL IL-1β group compared with those in control group respectively (P 0.05). MMP-9/ICAM-1 levels were significant decreased in 10 ng/mL IL-1β+ 10 μg/mL IL-1Ra group compared with those control group (P 0.05). They also had no significant difference in 1 ng/mL IL-1β+ 10 μg/mL IL-1Ra group compared with those in 100 ng/mL IL-1β+ 10 μg/mL IL-1Ra group (P>0.05). Conclusions Appropriate proportion of IL-1β and IL-1Rα can regulate embryo implantation. Key words: interleukin-1β; interleukin-1Rα; co-culture; matrix metalloproteinase-9; intercellular adhesion molecule-1