The affection of interleukin-1β and leptin on MMP9 and ICAM-1 in the embryo implantation

Objective:To study the mechanism of interleukin-1β(IL-1β) and leptin on embryo implantation in the early stage by observing the expression of intercellular adhesion molecule-1(ICAM-1) and metalloproteinase 9(MMP-9) intervened by IL-1β or leptin which were closely related to the implantation in the vial model of embryo and endometrium.Methods:The expressions of ICAM-1 and MMP-9 in the endometrium were detected by immunohistochemistry.However,the expressions of ICAM-1 and MMP-9 in the medium of model which have been intervened seperately by IL-1β,leptin or interleukin-1 receptor antagonist(IL-1ra) were detected by ELISA..Results:Immunohistochemistry:ICAM-1 was mainly expressed in the glandular cells,rarely in stromal cells,but MMP-9 was expressed in endometrial stromal cells and glandular cells.The result of ELISA showed that the expression of ICAM-1 in the medium was upregulated after intervened by IL-1β(10 ng/ml) or leptin(100 ng/ml)(P0.05).But after separately intervened by IL-1β(0.1 ng/ml,1 ng/ml),leptin(1,10 ng/ml) or IL-1ra(0.25,2.5,25 μg/ml),they had no significant difference compared with control group(P0.05).The result of ELISA showed that the expression of MMP-9 in the medium was upregulated after intervened by IL-1β(1,10 ng/ml) or leptin(1,10,100 ng/ml)(P0.05).But after separately intervened by IL-1β(0.1 ng/ml) or IL-1ra(0.25,2.5,25 μg/ml),they had no significant difference compared with control group(P0.05).Conclusion:Certain amounts of IL-1β and leptin can upregulate the expression of ICAM-1 and MMP-9.