2017•The International Encyclopedia of PrimatologyRequires access

Polymerase Chain Reaction ( PCR ), Quantitative/Real‐Time PCR ( qPCR ), Reverse Transcriptase PCR ( RT‐PCR )

Todd R. Disotell

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Abstract

The polymerase chain reaction (PCR) and its derivatives such as quantitative/real‐time PCR (qPCR) and reverse transcriptase PCR (RT‐PCR) have revolutionized molecular approaches to primatology. These techniques allow for the characterization of precise regions of DNA and RNA from minute samples and the determination of their levels of expression both qualitatively and quantitatively.

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The polymerase chain reaction (PCR) and its derivatives such as quantitative/real‐time PCR (qPCR) and reverse transcriptase PCR (RT‐PCR) have revolutionized molecular approaches to primatology. These techniques allow for the characterization of precise regions of DNA and RNA from minute samples and the determination of their levels of expression both qualitatively and quantitatively.

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Available abstract

The polymerase chain reaction (PCR) and its derivatives such as quantitative/real‐time PCR (qPCR) and reverse transcriptase PCR (RT‐PCR) have revolutionized molecular approaches to primatology. These techniques allow for the characterization of precise regions of DNA and RNA from minute samples and the determination of their levels of expression both qualitatively and quantitatively.

Key concepts: Real-time polymerase chain reaction, Reverse transcriptase, Polymerase chain reaction, Reverse transcription polymerase chain reaction, Molecular biology, Chemistry, Biology, Messenger RNA

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