2010•PubMedRequires access
[A real-time polymerase chain reaction-based test system for quantitation of Gumboro disease virus].
Pogoda Aa, Potekhina Ma
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Abstract
A real-time polymerase chain reaction-based test system for quantitation of infectious bursal disease (Gumboro disease) virus was developed. The reaction parameters were analyzed, which affected the linear relationship of a C1 depentanizer to the quantity of cDNA. The use of specific primers for reverse transcription was shown tohave some advantage over that of random hexanucleotides.