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APPLIED STUDY ON RAPID DETECTION OF INFLUENZA VIRUS A AND B BY FLUORESCENT REAL-TIME REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION

Fei Zhuo

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Abstract

[Objective]To explore the significance of detecting influenza viruses A and B by fluorescent real-time reverse transcriptase-polymerase chain reaction.[Methods]A total of 26 oral swab samples which were suspected of carrying influenza virus A and B were detected by fluorescent real-time RT-PCR and eggs assay.[Results]Out of 26 samples,detectable rate of fluorescent real-time RT-PCR was 15(57.7%) ,and detectable rate of eggs culture was 7(26.9%) .There was significant difference between them.[Conclusion]The fluorescent real-time PCR possess features of fast,reliable,higher sensitivity and higher specificity for detecting influenza viruses,which could be used as a rapid diagnostic method for detection of influenza viruses.

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What this paper is about

[Objective]To explore the significance of detecting influenza viruses A and B by fluorescent real-time reverse transcriptase-polymerase chain reaction.[Methods]A total of 26 oral swab samples which were suspected of carrying influenza virus A and B were detected by fluorescent real-time RT-PCR and eggs assay.[Results]Out of 26 samples,detectable rate of fluorescent real-time RT-PCR was 15(57.7%) ,and detectable rate of eggs culture was 7(26.9%) .There was significant difference between them.[Conclusion]The fluorescent real-time PCR possess features of fast,reliable,higher sensitivity and higher specificity for detecting influenza viruses,which could be used as a rapid diagnostic method for detection of influenza viruses.

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Available abstract

[Objective]To explore the significance of detecting influenza viruses A and B by fluorescent real-time reverse transcriptase-polymerase chain reaction.[Methods]A total of 26 oral swab samples which were suspected of carrying influenza virus A and B were detected by fluorescent real-time RT-PCR and eggs assay.[Results]Out of 26 samples,detectable rate of fluorescent real-time RT-PCR was 15(57.7%) ,and detectable rate of eggs culture was 7(26.9%) .There was significant difference between them.[Conclusion]The fluorescent real-time PCR possess features of fast,reliable,higher sensitivity and higher specificity for detecting influenza viruses,which could be used as a rapid diagnostic method for detection of influenza viruses.

Key concepts: Reverse transcriptase, Virology, Real-time polymerase chain reaction, Polymerase chain reaction, Reverse transcription polymerase chain reaction, Virus, Fluorescence, Biology

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APPLIED STUDY ON RAPID DETECTION OF INFLUENZA VIRUS A AND B BY FLUORESCENT REAL-TIME REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION — Research Paper | ScholarLens