Insecticidal Activity of Radish, Raphanus sativus Linn. (Brassicaceae) Roots Extracts.

Chemical constituents of Raphanus sativus Linn. roots were extracted and tested for their toxicity against adults and second instar nymphs of A. gossypii under laboratory conditions. The most effective fraction against both adults and nymphs was methylene chloride fraction with LC50 values of 386.63 and 309.43 ppm, followed by ethyl acetate fraction with LC50 values of 394.9 and 334.37 ppm then petroleum ether fraction with LC50 values of 636.2 and 424.56 ppm, for adults and nymphs, respectively. Also, the volatile components of each fraction were qualitatively and quantitatively identified and characterized by GC/MS technique. Moreover, the impact of the sub-lethal concentrations of R. sativus roots extracts on transaminases and alkaline phosphatase of A. gossypii was studied. Great inhibition of all tested enzymes activity was observed. This suggested the high potency of R. sativus roots extracts as environmentally friendly alternatives of traditional insecticides. Chemical constituents of Raphanus sativus Linn. roots were extracted and tested for their toxicity against adults and second instar nymphs of A. gossypii under laboratory conditions. The most effective fraction against both adults and nymphs was methylene chloride fraction with LC50 values of 386.63 and 309.43 ppm, followed by ethyl acetate fraction with LC50 values of 394.9 and 334.37 ppm then petroleum ether fraction with LC50 values of 636.2 and 424.56 ppm, for adults and nymphs, respectively. Also, the volatile components of each fraction were qualitatively and quantitatively identified and characterized by GC/MS technique. Moreover, the impact of the sub-lethal concentrations of R. sativus roots extracts on transaminases and alkaline phosphatase of A. gossypii was studied. Great inhibition of all tested enzymes activity was observed. This suggested the high potency of R. sativus roots extracts as environmentally friendly alternatives of traditional insecticides.