In vitro Biological ActivityAssociated with Extracts of Taiwanofungus camphoratus Spent Culture Broth

Spent broth from submerged cultures of Taiwanofungus camphorata grown at 28℃for seven days was concentrated 10-fold under vacuum at 45 ℃,extracted with four different organic solvents(petroleum ether,chloroform,ethyl acetate and n-butanol)and,after removing the organic solvents in a rotary evaporator,the residues were dissolved in DMSO.Petroleum ether,chloroform and ethyl acetate extracts at5μg/mL concentration(in DMSO)inhibited the proliferation of K562cells in vitro by 74%,72%and 66%,respectively compared with 68% inhibition recorded with 100μg/mL 5-flurouracil(5-Fu).LNCaP cell proliferation was inhibited 72%,73% and 69%,respectively by 25μg/mL concentrations of petroleum ether,chloroform and ethyl acetate extracts compared with 66% inhibition by 100μg/mL 5-Fu.NO production by RAW264.7macrophages was 12.12,13.75and 14.32μmol/106 cells following treatment with100μg/mL,200μg/mL and 500μg/mL concentrations of n-butanol extract respectively,significantly higher compared to 9.52μmol NO/106 cells released following treatment with PBS.All the extracts(except25μg/mL n-butanol extract)exhibited a capacity to repair damage to PC12cells induced by NaN3.