Tissue isolation and mycelium growth characteristics of Lactarius delicious

The tissues on different positions of 6 basidiocarps of different sources are isolated with 5 kinds of culture media .The best medium for tissue isolation of L .delicious is PDA culture medium and 8 well-developed mycelia are singled out .The research of growth characteristics of mycelium A1 indicates that the mycelia can absorb all the carbon sucrose tested .The sucrose is the best utilized carbon nutrition , the glucose is the better and the fructose is the worst .As for the utilization of nitrogen source , of organic nitrogen , single nitrogen is better than compound nitrogen ;and urea cant be utilized .Of inorganic nitrogen , ammonium nitrogen is better than nitrate nitrogen , ammonium sulfate has the best effect and nitrite cant be utilized .Mycelia have strong adaptability to salt concentration and can grow when the salt concentration is 14 gL-1 .The optimum salt concentration for mycelia growth ranges from 0 to 6 gL-1 .The range of temperature for growth is 7 ～ 37 ℃.The optimum temperature for mycelia growth ranges from 15 to 30 ℃.The optimum temperature for growth is 27 ℃.The mycelia prefer neutral and acid environment .The pH values suitable for growth is 5 ～ 7 and the optimum one is 6.5 .[ Ch , 2 fig .4 tab .7 ref .]