Simultaneous determination of 9 nucleosides analogues in Fritillaria anhuiensis by RP-HPLC.

[Objective] To develop reverse phase high performance liquid chromatography(RP-HPLC) method for simultaneous determination of 9 nucleosides and nucleobases in Fritillaria anhuiensis S.C.Chen et S.E.Yin.[Methods] The analyses were performed on an Agilent Zorbax SB-Aq C18(4.6 mm × 250 mm,5 μm)for all analyses.The investigated compounds were separated with gradient mobile phase consisting of water and Methanol.The flow rate was 1.0 ml/min.The detection wavelength was 260 nm with the 25 ℃ temperature of column.[Result] The investigated compounds including uracil,cytidine,uridine,hypoxanthine,inosine,guanosine,thymidine,adenosine,adenine had good linearity(r2 ≥ 0.998 2).The average recovery was 96.7% to 104.3% with RSD ≤ 3.87%.[Conclusion] The accuracy,stability,repeatability,and average recovery of the method are satisfying,and the 9 nucleosides and nucleobases of F.anhuiensis can be rapidly and accurately quantified by RP-HPLC.