Simultaneous determination of ten nucleoside and nucleobase components in Hulisan tablets by HPLC

Objective: To establish an HPLC method for simultaneous determination of ten nucleoside and nucleobase components in Hulisan tablets. Methods: The assay was performed on a Waters XselectTMHss C18column( 4. 6 mm ×250 mm,5 μm); the mobile phase consisted of methanol( phase A)- 0. 1% orthophosphoric acid water( phase B) with gradient elution at a flow rate of 0. 6 m L·min- 1; the column temperature was 30 ℃,and the detection wavelength was260 nm. Results: The investigated compounds including adenin,cytidine,hypoxanthine,xanthine,uridine,adenosine,2- deoxyuridine,guanosin,2- deoxidation inosine and thymidine showed good linearity( r≥0. 9994) in the ranges of9. 07- 181. 44,5. 03- 100. 50,2. 03- 40. 60,1. 54- 30. 87,3. 57- 71. 47,1. 25- 25. 08,3. 01- 60. 24,2. 02- 40. 48,0. 52- 10. 40 and 1. 05- 21. 00 μg·m L- 1,respectively. The average recoveries( n =6) ranged 96. 2%-103. 5% with RSD≤2. 6%. Conclusion: The HPLC method developed for simultaneous determination of nucleoside components was simple and valid. It could be used for quality evaluation and quality control of the Hulisan tablets.