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RP-HPLC simultaneous determination of five nucleosides in Radix Rehmanniae Preparata

Sun Li-xin

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Abstract

Objective: To establish a RP-HPLC method for simultaneously determining five nucleosides(hypoxanthine,uridine,adenine,guanosine and adenosine)in Radix Rehmanniae Preparata.Methods: The Diamonsil C18(4.6 mm×250 mm,5 μm)column was adopted and diluted with acetonitrile-0.04 mol·L-1 potassium dihydrogen phosphate gradiently at the wavelength of 254 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 30 ℃.Results: The method was developed with a good linearity within the range of 1.0-16.0 μg·mL-1(r=0.9999),5.0-80.0 μg·mL-1(r=0.9999),1.0-16.0 μg·mL-1(r=0.9997),1.25-20.0 μg·mL-1(r=0.9999)and 1.0-16.0 μg·mL-1(r=0.9999)for hypoxanthine,uridine,adenine,guanosine and adenosine,respectively.And the average recoveries of the five nucleosides were 98.1%-101.0%.Conclusion: The established method can be used for the quantification of five nucleosides(hypoxanthine,uridine,adenine,guanosine and adenosine)in Radix Rehmanniae Preparata.

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Objective: To establish a RP-HPLC method for simultaneously determining five nucleosides(hypoxanthine,uridine,adenine,guanosine and adenosine)in Radix Rehmanniae Preparata.Methods: The Diamonsil C18(4.6 mm×250 mm,5 μm)column was adopted and diluted with acetonitrile-0.04 mol·L-1 potassium dihydrogen phosphate gradiently at the wavelength of 254 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 30 ℃.Results: The method was developed with a good linearity within the range of 1.0-16.0 μg·mL-1(r=0.9999),5.0-80.0 μg·mL-1(r=0.9999),1.0-16.0 μg·mL-1(r=0.9997),1.25-20.0 μg·mL-1(r=0.9999)and 1.0-16.0 μg·mL-1(r=0.9999)for hypoxanthine,uridine,adenine,guanosine and adenosine,respectively.And the average recoveries of the five nucleosides were 98.1%-101.0%.Conclusion: The established method can be used for the quantification of five nucleosides(hypoxanthine,uridine,adenine,guanosine and adenosine)in Radix Rehmanniae Preparata.

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Available abstract

Objective: To establish a RP-HPLC method for simultaneously determining five nucleosides(hypoxanthine,uridine,adenine,guanosine and adenosine)in Radix Rehmanniae Preparata.Methods: The Diamonsil C18(4.6 mm×250 mm,5 μm)column was adopted and diluted with acetonitrile-0.04 mol·L-1 potassium dihydrogen phosphate gradiently at the wavelength of 254 nm.The flow rate was 1.0 mL·min-1 and the column temperature was 30 ℃.Results: The method was developed with a good linearity within the range of 1.0-16.0 μg·mL-1(r=0.9999),5.0-80.0 μg·mL-1(r=0.9999),1.0-16.0 μg·mL-1(r=0.9997),1.25-20.0 μg·mL-1(r=0.9999)and 1.0-16.0 μg·mL-1(r=0.9999)for hypoxanthine,uridine,adenine,guanosine and adenosine,respectively.And the average recoveries of the five nucleosides were 98.1%-101.0%.Conclusion: The established method can be used for the quantification of five nucleosides(hypoxanthine,uridine,adenine,guanosine and adenosine)in Radix Rehmanniae Preparata.

Key concepts: Chemistry, Uridine, Hypoxanthine, Chromatography, Guanosine, High-performance liquid chromatography, Adenosine, Nucleoside

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