Allelic variation at HMW-GS of Glu-1 in the latest wheat breeding lines and landraces from Huanghuai area of China

【Objective】 Variation of high molecular weight glutenin subunit(HMW-GS) at the Glu-1 was studied in the latest 111 wheat breeding lines and 39 wheat landraces of Huanghuai area of China.【Method】 HMW-GS composition was determined by sodium-dodecyl-sulphate polyacrylamide gel electrophoresis(SDS-PAGE).【Result】 For the latest wheat breeding lines,ten alleles were identified at the Glu-1 locus,19 different HMW-GS patterns were detected.Three major genotypes of HMW-GS:1,7+9,2+12;1,7+9,5+10 and null,7+9,2+12 were found at higher frequencies of 20.7%,10.8% and 9.9%,respectively.For the wheat landraces,seven alleles were identified at the Glu-1 locus,5 different HMW glutenin subunit patterns were detected.The most frequent HMW-GS pattern was null,7+8,2+12(90.0%).One subunit pair 7**+8** at Glu-B1 locus,which has never been reported,was found in landrace Banjiemang.【Conclusion】 The results showed the 50 years evolution of wheat breeding in Huanghuai area is useful in breeding programs to improve bread making quality,develop the HMW-GS that is related to good bread-making quality,such as 1 and 5+10.Other types such as 2*(Glu-A1b),13+16(Glu-B1f) and 17+18(Glu-B1i)are very poor in this area.