Genetic Diversity of High Molecular Weight Glutenin Subunit (HMW-GS) Composition in Common Wheat Landraces from Xinjiang,China

The high molecular weight glutenin subunit (HMW-GS) composition of 282 common wheat (Triticum aestivum L.) landraces originated from Xinjiang Uygur Autonomous District were analyzed by sodium dodecyl sulphate polyacrylamide-gel electrophoresis (SDS-PAGE) method. The numbers of alleles on Glu-A1, Glu-B1 and Glu-D1 loci were 3, 6 and 5 respectively. The predominant subunits at the Glu-A1, Glu-B1 and Glu-D1 loci were null, 7 + 8 and 2 + 12, and their frequencies were 75.5%, 90.8% and 72.0%, respectively. A total of 20 kinds of HMW-GS combinations were observed in the 282 common wheat landraces tested. The most frequent combination was (null, 7+8, 2+12) (52.8%), followed by combinations (null, 7 + 8, 2.6 + 12) (14.1%) and (2*, 7 + 8, 2 + 12) (11.0%). The frequencies of other combinations were all less than 10%. In addition, a novel subunit 2.6+12 at the Glu-D1 locus was detected in this study. In the 282 common wheat landraces tested, two landraces harboring high processing quality HMW-GS combination, (2*, 7 + 9, 5 + 10) and (1, 7 + 9, 5 + 10) were identified. These materials may be used as potential genetic resources for improving processing quality in a wheat breeding program.