Optimization of extraction process for polysaccharide from Hippophae fhamnoides L.

[Objective] To optimize the extraction process of polysaccharide extracted with ultrasonic-microwave synergetic extraction from Hippophae fhamnoides L..[Method] By employing both the single factor test and the orthogonal test,the optimal parameters of ultrasonic-microwave synergetic extraction for the extraction of polysaccharide from Hippophae fhamnoides L.were confirmed,and the extraction efficiency was comparatively studied with that of water extraction,microwave extraction and ultrasonic extraction.[Result] The optimum extraction processes of ultrasonic-microwave synergetic extraction were as follows:180 s of extraction duration,450 W of extraction power and 1:30 of solid-liquid ratio(W/V).The yield of polysaccharide extracted from Hippophae fhamnoides L.under the optimum conditions reached 22.50 mg/g.[Conclusion] The ultrasonic-microwave synergistic extraction for the extraction of polysaccharide from Hippophae fhamnoides L.was superior to water extraction,microwave extraction and ultrasonic extraction.