Study on Extraction of Polysaccharide from Hippophae fhamnoides L. by Ultrasonic-Microwave Synergistically Assisted Technique

[Objective]The study aimed to optimize the ultrasonic-microwave synergistic extraction technique of polysaccharide from Hippo-phae fhamnoides L..[Method]The effects of extraction time,power of microwave and the ratio of liquid to solid on the yield of polysaccharide were explored by single-factor experiment,and the optimum technique parameter was determined by the orthogonal test.And then the extraction effect of the method was compared with that of water extraction,microwave assisted extraction and ultrasonic assisted extraction.[Results]The optimal technique conditions for the ultrasonic-microwave synergistic extraction were:the extraction time 180 s,power of microwave was 450 W and the ratio of solid to liquid 1∶30,under these conditions,the highest yield of polysaccharide from Hippophae fhamnoides L.was 22.50 mg/g.[Conclusion]The ultrasonic-microwave synergistic extraction is better than other extraction methods.