Influence on the hepatic ischemia-reperfusion injuries by ethanol preconditioning and its relationship with HSP70 induction

Objective To explore the influence on 90-min ischemia-reperfusion liver injuries by ethanol preconditioning and its relationship with hepatic HSP70 induction.Methods The Wistar rats were pretreated under the dose of 5 g/kg of 40%.The ischemia-reperfusion injuries were performed under the mode of hepatic blood inflow occlusion with caudate lobe bypass.232 adult male Wistar rats were randomly divided into 5 groups:normal control group,ethanol group,ischemia/reperfusion group,NS pretreated group,ethanol pretreated group.Ischemia period was set at 90 min.The mortality,changes of ALT/AST activities,pathologic changes and HSP70/HSP72mRNA level in the liver were determined in different groups.Results After 90-min I/R injuries,in the ethanol pretreated group,a good liver structure and less changes of ALT/AST activities can be easily found,also with more hepatic HSP70 expression.Conclusions the enhanced protection from the 90 min I/R injuries can be got in the rat liver by ehanol precondition,and HSP70 maybe the underlied protective substance elucited by ethanol precondition to fight against the liver I/R injuries.