STUDIES ON ISOLATION, PURIFICATION AND ANTITUMOR ACTIVITY OF POLYSACCHARIDES OF PLEUROTUS FERULAE

PFW was isolated from fruit body of Pleurotus ferulae by using hot water Proteins were removed from PFW with Sevag method, after DEAE cellulose chromatography, PF 1 and PF 2 were isolated from PFW The weight was determined by using multiangle laser photometer combined with GPC; the results showed: PF 1 contains two fractions, the weights (Mw) are 7 875×10 5 and 3 245×10 4, the Mw of PF 2 is 3 187×10 6 The components and structure of polysaccharides were analyzed by IR and GC The polysaccharides PF 1 consists of rhamnose, glucose, galactose, mannose; PF 2 consists of rhamnose, xylose, glucose, galactose, mannose; PF 1 contains β glucoside and mannoside, PF 2 contains α glucoside It was found polysaccharides have antitumor activity on the implanted Sarcoma 180 in mice