2006•Shanghai nongye xuebaoRequires access
Establishment of DNA fingerprints on two cabbage hybrids of "Xiaguang" and "Zaoxia 16"
Chong Liu, Shuxia Wang, Yunying Ren
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Abstract
The genomic DNAs were extracted by the method of SDS from two cabbage(Brassica oleracea var.capitata) hybrids(Xiaguang,Zaoxia 16) and their parents in order to establish their DNA fingerprints by RAPD and SSR methods for identification of seed purity.The genomic DNAs of each hybrid and its parents were used as one group of template DNA to screen 30 pairs of SSR primers and 50 RAPD primers.The results indicated that the types of DNA bands amplified by most SSR primers in the two groups was similar,so the establishment of SSR fingerprints failed.But 3 efficacious RAPD primers were gained in each group,which were S15,S42,S147 and S42,S78,S88 respectively.The specific RAPD fingerprints in the two groups could be established with S42 primer and then transformed into digital fingerprints.