2002Chinese Journal of Oil Crop ScievesRequires access

Fingerprinting analysis of hybrid parents in Brassica napus based on RAPD

Duan Yuansheng, Pingwu Liu, Yang Guangsheng

Open publisher page 0 citations

Abstract

random primers were used for PCR amplifications with 39 hybrid parents in Brassica napus , and 40 primers were chosen to further PCR amplifications for them producing main bands with good repeatility. Finally 9 best primers were selected for fingerprinting analysis in this study. The fingerprints obtained from these 9 primers could distinguish 27 materials and the other four groups consisting of 12 materials. The materials in each group were sister materials. The fingerprints of these 39 materials were consistent with their pedigree.

About this research paper

What this paper is about

random primers were used for PCR amplifications with 39 hybrid parents in Brassica napus , and 40 primers were chosen to further PCR amplifications for them producing main bands with good repeatility. Finally 9 best primers were selected for fingerprinting analysis in this study. The fingerprints obtained from these 9 primers could distinguish 27 materials and the other four groups consisting of 12 materials. The materials in each group were sister materials. The fingerprints of these 39 materials were consistent with their pedigree.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

random primers were used for PCR amplifications with 39 hybrid parents in Brassica napus , and 40 primers were chosen to further PCR amplifications for them producing main bands with good repeatility. Finally 9 best primers were selected for fingerprinting analysis in this study. The fingerprints obtained from these 9 primers could distinguish 27 materials and the other four groups consisting of 12 materials. The materials in each group were sister materials. The fingerprints of these 39 materials were consistent with their pedigree.

Key concepts: Brassica, RAPD, DNA profiling, Genetics, Biology, Botany, DNA, Genetic diversity

Related papers

Back to paper searchBrowse research topicsOriginal source
Fingerprinting analysis of hybrid parents in Brassica napus based on RAPD — Research Paper | ScholarLens