Fingerprinting analysis of hybrid parents in Brassica napus based on RAPD
Duan Yuansheng, Pingwu Liu, Yang Guangsheng
Abstract
Duan Yuansheng, Pingwu Liu, Yang Guangsheng
Abstract
random primers were used for PCR amplifications with 39 hybrid parents in Brassica napus , and 40 primers were chosen to further PCR amplifications for them producing main bands with good repeatility. Finally 9 best primers were selected for fingerprinting analysis in this study. The fingerprints obtained from these 9 primers could distinguish 27 materials and the other four groups consisting of 12 materials. The materials in each group were sister materials. The fingerprints of these 39 materials were consistent with their pedigree.
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random primers were used for PCR amplifications with 39 hybrid parents in Brassica napus , and 40 primers were chosen to further PCR amplifications for them producing main bands with good repeatility. Finally 9 best primers were selected for fingerprinting analysis in this study. The fingerprints obtained from these 9 primers could distinguish 27 materials and the other four groups consisting of 12 materials. The materials in each group were sister materials. The fingerprints of these 39 materials were consistent with their pedigree.
Key concepts: Brassica, RAPD, DNA profiling, Genetics, Biology, Botany, DNA, Genetic diversity