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2004•Journal of Yanan UniversityRequires access

Single sweep oscillopolarographic determination of chloramphenicol

Yuanhua Yang

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Abstract

Linear-sweep polarography was used in the present research to study the electrochemical behavior of chloramphenicol(CAP).In a supporting electrolyte containing 0.3 mol/LKCl(pH7.30),a reduction peak of chloramphenicol was observed at Hg electrode. The peak potential was -0.620 V(vs.SCE).The relationship between peak current and the concentration of CAP was linear in the rane of 1×10~(-7) mol/L～9×10~(-6) mol/L(r=0.9992). The detection limit was 5.0×10~(-8) mol/L.The method was applied to determination of chloramphenicol in urine sample and tablet with satisfactory results.

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What this paper is about

Linear-sweep polarography was used in the present research to study the electrochemical behavior of chloramphenicol(CAP).In a supporting electrolyte containing 0.3 mol/LKCl(pH7.30),a reduction peak of chloramphenicol was observed at Hg electrode. The peak potential was -0.620 V(vs.SCE).The relationship between peak current and the concentration of CAP was linear in the rane of 1×10~(-7) mol/L～9×10~(-6) mol/L(r=0.9992). The detection limit was 5.0×10~(-8) mol/L.The method was applied to determination of chloramphenicol in urine sample and tablet with satisfactory results.

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Available abstract

Linear-sweep polarography was used in the present research to study the electrochemical behavior of chloramphenicol(CAP).In a supporting electrolyte containing 0.3 mol/LKCl(pH7.30),a reduction peak of chloramphenicol was observed at Hg electrode. The peak potential was -0.620 V(vs.SCE).The relationship between peak current and the concentration of CAP was linear in the rane of 1×10~(-7) mol/L～9×10~(-6) mol/L(r=0.9992). The detection limit was 5.0×10~(-8) mol/L.The method was applied to determination of chloramphenicol in urine sample and tablet with satisfactory results.

Key concepts: Chloramphenicol, Polarography, Linear sweep voltammetry, Detection limit, Chemistry, Electrolyte, Chromatography, Linear relationship

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