The comparative analysis of lymphocyte subpopulations in healthy children and adults in Guangzhou

Objective:To compare the similarities and differences for the percentage and absolute counts of lymphocyte subsets in healthy children and adults and provide the reference values for clinical diagnosis and treatment in Guangzhou.Methods: The number of T lymphocyte cell subsets(CD3+cells,CD3+CD4+ cells,CD3+CD8+cells),B lymphocyte cells(CD19+) and natural killer cells(CD3-CD56+ NK cells) in peripheral blood were tested by Two-color flow cytometric.Results: Absolute counts and percentage of total lymphocyte cell and CD19+ lymphocyte cell,percentage of CD3+ lymphocyte cell and CD3+CD4+ lymphocyte cell,absolute counts of CD3+CD8+ lymphocyte cell and CD3-CD56+ cell and CD4+/CD8+cell value have significantly different between children and adult group.The percentage of CD3+CD8+ lymphocyte cell and CD3-CD56+ cell and absolute counts of CD3+ lymphocyte cell and CD3+CD4+ lymphocyte cell have no significantly defferent between the two groups.The percentage and absolute counts of CD3+CD8+ lymphocyte cell,CD19+ lymphocyte cell and CD3-CD56+ cell were higher and the percentage and absolute counts of CD3+ lymphocyte cell,CD3+CD4+ lymphocyte cell and CD4+/CD8+cell value were lower in children group than adult group.Several index have significantly different in the same or different gender.Conclusion:The peripheral blood lymphocyte subpopulations values differ by different age and gender.The changes in the percentage and absolute counts of lymphocyte subpopulations are not always consistent with changes in their relative ages.The absolute counts of lymphocyte subpopulations as a reference values for diagnosis of hematologic and immunologic disorders rather than the percentage.