Cloning,Prokaryotic Expression and Antiviral Activity of Chicken Interferon-α Gene

The full length of chicken interferon alpha(ChIFN-α)gene was amplified by polymerase chain reaction(PCR)from the genome DNA of Luoman chicken liver,and then sequenced.The amplified gene fragment was 582 bp,of which the 489 bp coding region was subcloned into prokaryotic expression vector pQE30.The recombinant plasmid pQEChIFN-α was identified by PCR,enzyme digestion and DNA sequencing.SDS-PAGE and Western blot showed that a 19.80 kDa fusion protein was expressed in the form of inclusion body with good immunity.After being denatured and renatured,the activity of inclusion body was detected by inhibiting the cytopathic effect.The activity of recombinant ChIFN-α against vesicular stomatitis virus at Vero cell is 1.16×103 U/mg.