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2009•Zhongguo yufang shouyi xuebaoRequires access

Prokaryotic expression and activity assay of recombinant chicken interferon-alpha.

Hua Dai, Jiayu Zheng, Jun-Hua Chen, Pan Zhiming, Jiao Xin-an

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Abstract

Chicken IFN-αgene was amplified by RT-PCR and cloned from chicken spleen lymphocytes stimulated by concanavalin A.Recombinant plasmids (pGEX-proChIFN-α,pET-ChIFN-α) were expressed in E.coli.SDS-PAGE analysis showed that the expression products had a molecular weight of 45 ku and 25 ku respectively,and existed in inclusion bodies.The expressed protein was shown to inhibit H5N1 avian influenza virus or vesicular stomatitis virus replication on chick embryo fibroblast (CEF) at 2×106 u/mg.The potential of chicken IFN-α as adjuvant was discussed.

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What this paper is about

Chicken IFN-αgene was amplified by RT-PCR and cloned from chicken spleen lymphocytes stimulated by concanavalin A.Recombinant plasmids (pGEX-proChIFN-α,pET-ChIFN-α) were expressed in E.coli.SDS-PAGE analysis showed that the expression products had a molecular weight of 45 ku and 25 ku respectively,and existed in inclusion bodies.The expressed protein was shown to inhibit H5N1 avian influenza virus or vesicular stomatitis virus replication on chick embryo fibroblast (CEF) at 2×106 u/mg.The potential of chicken IFN-α as adjuvant was discussed.

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Available abstract

Chicken IFN-αgene was amplified by RT-PCR and cloned from chicken spleen lymphocytes stimulated by concanavalin A.Recombinant plasmids (pGEX-proChIFN-α,pET-ChIFN-α) were expressed in E.coli.SDS-PAGE analysis showed that the expression products had a molecular weight of 45 ku and 25 ku respectively,and existed in inclusion bodies.The expressed protein was shown to inhibit H5N1 avian influenza virus or vesicular stomatitis virus replication on chick embryo fibroblast (CEF) at 2×106 u/mg.The potential of chicken IFN-α as adjuvant was discussed.

Key concepts: Biology, Recombinant DNA, Molecular biology, Vesicular stomatitis virus, Virology, Fowlpox virus, Virus, Concanavalin A

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