Fusion Expression and Construction of Antigen Epitopes from F Gene of Newcastle Disease Virus with Chicken Interferon-gamma

[Objective] The aim was to study the immune adjuvant effects of chicken interferon-γ(ChIFN-γ),a recombinant plasmid with ChIFN-γ and two antigen epitopes from F gene of Newcastle disease virus(NDV F2-3) was built and expressed in prokaryotic cells.[Method] Using enzyme digestion,chicken interferon-γ was inserted into pET-32a-NDV F2-3 expression vector,and then the recombinant pET-IFN-F was constructed and confirmed with PCR amplification,double restriction digestion and DNA sequencing transformed.The recombinant plasmid was expressed in E.coli BL21though IPTG.SDS-PAGE and Western blot were used to detect expression products.[Result] The length of recombinant gene was 726 bp by DNA sequencing.The fusion protein was about 35 000 and had the reactinogenicity with specific antibody.[Conclusion] The fusion gene encoding ChIFN-γ-NDV F2-3 could effectively express in prokaryotic cells and had a certain immune activity.