2005•Tianjin Yike Daxue xuebaoRequires access
Cloning and expression of recombinant human angiostatin
Ruimin Wang, Guangming Li, Qiuxiang Li
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Abstract
To clone the human angiostatin gene into a secretory-fusion vector, then to express. The total RNA was isolated from human hepatocytes. The angiostatin gene was synthesized and amplified from the mRNA by RT-PCR and the PCR product was cloned into pEZZ18 vector, then to express in E.coli DH5#alpha#. The angiostatin gene was successfully cloned and testified there was recombinant fusion angiostatin in E.coli DH5#alpha#. The experiment lays a foundation for tumor-antiangiogenesis drug therapy.