2004•Europe PMC (PubMed Central)Requires access
Cloning expression and purification of recombinant thymosin #alpha#_(1)
Zhaolong Gong, Zuo-jun Jiang, Yihui Xu, Jilong Shen, Wei Dong, Zhaojun Duan
Open publisher page 0 citations
Abstract
The fusion gene composed of Ref (a sort of T7 phage protein) and T#alpha#_(1) gene was synthesized, and the gene was cloned into expression vector pBV220, E.coliDH5#alpha# was transformed with recombinant plasmid and induced by temperature. The expressed fusion protein could be purified by CM-Sepharose Fast Flow cation exchange chromatography and identified by Western-blot. The purified fusion protein, Ref-T#alpha#_(1) was obtained. The molecular weight was about 15000. To express short peptide, the method of expressing fusion gene is practical. The successful expression of Ref-T#alpha#_(1) has established a foundation for biological activity research.