ScholarLens Back to search
2001•PROGRESS IN BIOCHEMISTRY AND BIOPHYSICSRequires access

Cloning and Expression of the Intracellular Cytoplasmic Domain of Rat Notch 1 Receptor

Weilin Jin, Baisong Liao, Ju Gong

Open publisher page 1 citations

Abstract

The cloned NICD fragment was confirmed by sequencing and then subcloned into glutathione-S-transferase (GST) fusion protein expression vector pGEX-KG. The GST-NICD fusion proteins were expressed in E. coli JM109 after inducing by IPTG. The fusion proteins were purified by affinity chromatography on glutathione Sepharose 4B.

About this research paper

What this paper is about

The cloned NICD fragment was confirmed by sequencing and then subcloned into glutathione-S-transferase (GST) fusion protein expression vector pGEX-KG. The GST-NICD fusion proteins were expressed in E. coli JM109 after inducing by IPTG. The fusion proteins were purified by affinity chromatography on glutathione Sepharose 4B.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The cloned NICD fragment was confirmed by sequencing and then subcloned into glutathione-S-transferase (GST) fusion protein expression vector pGEX-KG. The GST-NICD fusion proteins were expressed in E. coli JM109 after inducing by IPTG. The fusion proteins were purified by affinity chromatography on glutathione Sepharose 4B.

Key concepts: Fusion protein, lac operon, Affinity chromatography, Molecular biology, Cloning (programming), Sepharose, Glutathione S-transferase, Glutathione

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and Expression of the Intracellular Cytoplasmic Domain of Rat Notch 1 Receptor — Research Paper | ScholarLens