Effect of high concentration glucose and advanced glycation end products on MIP-1#alpha# mRNA expression in cultured human umbilical vein endothelial cells

To investigate the effect of high concentration glucose and advanced glycation end products (AGEs) on macrophage inflammatory protein-la (MlP-1#alpha#) mRNA expression in cultured human umbilical vein endothelial cells (HUVECs). HUVECs were cultured with AGEs alone at different concentrations (100, 200, or 400mg/L) for 24h and then co-cultured with 22mmol/L glucose and 400mg/L AGEs for 24h. The levels of MIP-l#alpha# mRNA expression in cultured HUVECs were detected by in situ hybridization. In situ hybridization showed that after exposure of HUVECs to AGEs alone at different concentrations (100, 200, 400mg/L) for 24h, the average integrated optical density values of MIP-1#alpha# mRNA expression in HUVECs were 18.76±3.17, 26.58±1.61 and 34.23 + 2.25 (BSA group, 13.83±1.24), with statistically significant differences between the groups (P<0.05). After exposure of HUVECs to 400mg/L AGEs and 22mmol/L glucose for 24h, the average integrated optical density values of MIP- 1#alpha# mRNA expression were 40.56±1.97, also with statistically significant differences between the groups (P<0.05). AGEs can promote the MIP-1#alpha# mRNA expression in cultured HUVECs in a dose-dependent manner, and there was a synergism between AGEs and high concentration glucose.