Effect of glucose on protein and mRNA expression of macrophage inflammatory protein-1α in cultured human umbilical vein endothelial cells

Objective: To study the effect of glucose concentration on protein and mRNA expression of macrophage inflammatory protein-1α(MIP-1α) in cultured human umbilical vein endothelial cells(HUVECs). Methods:HUVECs were cultured with glucose at different concentrations(5.6,11.1,16.7,22.0 and 28.0 mmol/L) for 10 days and at concentration of 22.0 mmol/L for 0, 5, 10, and 15 days .The levels of mRNA and protein expression of MIP-1α in cultured HUVECs were detected by situ hybridization and Western Blot. Results: Expression of MIP-1α increased with the improvement of glucose concentrations. When glucose concentration arrived 22.0 mmol/L, expression of MIP-1α was the most; when glucose concentration arrived 28.0 mmol/L, expression of MIP-1α decreased. The average integrated optical density values of MIP-1α mRNA in HUVECs were 12.54±2.78,17.69±1.38,23.35±2.13,32.23±2.25及28.46±2.46(P0.05). Expression of MIP-1α protein in HUVECs resulted in a 1.42-fold, a 1.87-fold,a 2.58-fold, and a 2.12-fold increase compared with the 5.6 mmol/L glucose group(P0.05).After exposure of HUVECs to 22.0 mmol/L glucose for 0,5,10, and 15 days, expression of MIP-1α increased. After 15 days, expression of MIP-1α was the most. The average integrated optical density values of MIP-1α mRNA expression in HUVECs were 10.43±2.13, 18.67±1.46,32.23± 2.25, and 38.28±3.14 (P0.05). Expression of MIP-1α protein in HUVECs resulted in a 1.75-fold, a 3.06-fold, and a 3.64-fold increase compared with the 0-day group(P0.05). Conclusion:Glucose could induce high expressions of MIP-1α mRNA and protein in cultured HUVECs in dose-dependent and time-dependent manner.