High Concentration of Glucose Induced NADPH Oxidase 4 Expression of HUVECs

Objective To investigate the change of NOX4 mRNA expression in HUVECs stimulated by high concentration of glucose.Methods Morphology of HUVECs was observed by inverted microscope,expression of factor Ⅷ related antigen in HUVECs was investigated by immunohistochemistry.HUVECs were divided into three groups:control group(treated with physiological concentration of glucose for 16 hours),high glucose group(treated with 30 mmol/L glucose for 16 hours) and DPI intervention group(treated with 30 mmol/L glucose plus 10μmol/l DPI for 16 hours). mRNA expression of NOX4 was detected by RT-PCR.Results The results showed that there was expression of factor Ⅷ related antigen in HUVECs.mRNA expression of NOX4 was increased in HUVECs stimulation with high glucose(P0.05,vs control group).After treated with DPI,mRNA expression of NOX4 in HUVECs decreased(P0.05,vs control group).Conclusions The mRNA expression of NOX4 in HUVECs increased significantly stimulated by high glucose,while DPI could decrease NOX4 mRNA expression in HUVECs