In vitro Skin Irritation Test of Honeypolis using Human Skin Model

Ethanol extracted propolis (EEP) was mixed with honey (honeypolis) to dissolve well in water and in vitro skin irritation test was conducted. In vitro method is designed to predict and classify the skin irritation potential of a chemical by assessment of its effect on EpiDermSUPTM/SUP, a reconstituted threedimensional human epidermis model. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT after a 60 min exposure period. In this study under the given conditions honeypolis showed no irritant effects. Honeypolis meets acceptance criteria if: mean absolute OD 570 nm of the three negative control tissues is ≥0.8 and ≤2.8, mean relative tissue viability of the three positive control tissues is ≤ 20%, standard deviation of relative tissue viability obtained from each three concurrently tested tissues is ≤ 18%. Honeypolis is therefore classified as “non-irritant” in accordance with UN GHS “No Category”.