Separation and Determination of Cordycepin and Adenosine in Artificial Cordyceps Sinensis Mycelium by Gradient UPLC

Uracil, guanine, adenine, guanosine, adenosine, cordycepin are the most important bases in Cordyceps sinensis, but the separation and analysis of this compound is not reported. After optimizing the composition of mobile phase, program of gradient elution and flow rate, the essay used methanol-water as mobile phase, gradient elution way, flow rate 0.3mL/min, detected at 260nm, the injection volume was 3μL, column temperature was 40°C. The six matters could be easily determined in 10min by UPLC. This method is used to determinate the content of the bases in the artificial cordyceps, results show that the method is simple, rapid and high sensitivity, reproducible and practical value.