Analysis of adenosines in anamorphic mycelia of several species of Cordyceps

Spectrophotometer was used to examine different extraction method of adenosine components from anamorphic mycelia of Cordyceps species.Thin layer chromatography and high performance liquid chromatography were used to analyze qualitatively and quantitatively the adenosine components including adenine,adenosine,cordycepin and N6-(2-hydroxyethyl)-adenosine(HEA).The results showed that using distilled water ultrasonic treatment for 30min was the best extraction method.The data showed that five examined species of Cordyceps could produce adenosine and adenine.Cordyceps militaris produced cordycepin,and Cordyceps pruinosa and Cordyceps cicadae produced HEA.It was found that the incubation duration could affect the accumulation of HEA in the anamorphic mycelia.