2009•Journal of Pathogen BiologyRequires access
Establishment of real-time fluorescent PCR for detection of Campylobacter jejuni
Lianfeng Du, Sun Wanbang, Yulin He, Yu Yan, Yang Rui, Song Ming-ying
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Abstract
Objective To establish a rapid and accurate method for detection of Campylobacter jejuni by real-time fluorescent PCR assay.MethodsAccording to the open reading frame (ORF) of sequence of flaA gene, gyrA gene, several couples of specific primers were designed.Reaction system and determination approach of real-time fluorescent PCR were established for detection of C. jejuni.ResultsThe flaA primer pairs were used in real-time PCR assay for detection of C. jejuni, the fluorescent amplification curves were observed as a typical S curve, other bacteria were also tested and no amplification was observed.It was found that the sensitivity of this assay was 10 CFU/ml.ConclusionThis real-time PCR assay is fast, simple, sensitive and specific for the purposes of detection of C. jejuni.