Detection of Campylobacter jejuni by real-time fluorescent PCR

Objective To explore a rapid,sensitivety and specific method for examining Campylobacter jejuni(C.jejuni).Methods According to C.jejuni sequences from Ganbank,the specific primers and probe were designed on the basis of conserved gene region and a TaqMan real-time fluorescent polymerase chain reaction(PCR) was established.The specificity,sensitivity and repeatability of the assay were also studied.Results C.jejuni was amplified by real-time fluorescent PCR successfully while all non-C.jejuni bacterial species did not generate any signal.The assay had high sensitivity and repeatability,and the low detecting limit was 10 cfu /mL.Conclusions The real-time fluorescent PCR is a rapid,specific and sensitive method for quantifying C.jejuni,thus it has high practical value of application.