Application of real-time PCR for quantitative detection of Campylobacter jejuni by TaqMan probe

Campylobacter jejuni is recognized as a leading human food_borne pathogen.Traditional biochemical identification forC.jejuni is not reliable due to special growth requirements and the possibility that this bacterium can enter a viable but nonculturable(VNC) state.Nucleic acid_bassed tests have emerged as a useful alternative to traditional testing.In this article,Based LigntCycler we present fluorescent quantitative PCR assay for quantitative detection of C.jejuni.When this assay was applied,the assay positive for all of the isolates of C.jejuni tested(11 iolates,including type strain ATCC33560) and negative for all other Campylobacter spp(3 isolates) and several other bacteria (5 species tested).The total assa could be completed in 60 min with a detection limit of approximately 2 CFU,and a correlation coefficient was 0.988.Result indicated that fluorescent quantitative detection mehtods private not only a special,sensitive,rapid method for quantitative detection of c.jejuni,but also a important method for researching mechanism pathogenesis of C.jejuni.