Study on Teratogenic Effect of Nitrobenzene on Vicia faba Root Tip Cells

The mitotic index, micronucleus rate and chromosome aberration of Vicia faba root tip cells were determined by using micronucleus test and chromosome aberration test method of Vicia faba root tip cells with different concentrations of nitrobenzene as mutagens rate. The results showed that nitrobenzene could induce the higher frequency of micronucleus, and the micronucleus rate increased with the increase of nitrobenzene concentration at 4 and 24 h, but decreased after a certain concentration. Different concentrations of nitrobenzene at different treatment time to make Vicia faba root tip cells produce higher frequency chromosome aberration, and produce a variety of types of chromosomal aberrations. Therefore, nitrobenzene has obvious teratogenic effect on Vicia faba root tip cells.