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2004•Journal of environmental healthRequires access

The Effects of Lead on Root Tip Cell of Vicia faba

Zheng Guo-zh

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Abstract

Objective To study the genetic effects of lead on root tip cell of Vicia faba. Methods Micronucleus tests were conducted on root tip cell of Vicia faba treated with Pb2+ of concentrations of 0.1, 1.0, 5.0, 25.0, 50.0 mg/L for 12-36 h. Results The results indicated that the rates of micronucleus of lead-treated tip cell were significantly higher than that of the control (P0.01),the mitotic index significantly decreased compared with the control (P0.05)and the response of high dose-short time exposure was the same as that of low dose-long time exposure. Conclusion Lead may induce, concentration-dependent and time-dependent, rate of micronucleus increasing in Vicia faba root tip cell and mitotic index decreasing as well. It is feasible to monitor lead pollution using micronucleus test on root tip cell of Vicia faba.

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What this paper is about

Objective To study the genetic effects of lead on root tip cell of Vicia faba. Methods Micronucleus tests were conducted on root tip cell of Vicia faba treated with Pb2+ of concentrations of 0.1, 1.0, 5.0, 25.0, 50.0 mg/L for 12-36 h. Results The results indicated that the rates of micronucleus of lead-treated tip cell were significantly higher than that of the control (P0.01),the mitotic index significantly decreased compared with the control (P0.05)and the response of high dose-short time exposure was the same as that of low dose-long time exposure. Conclusion Lead may induce, concentration-dependent and time-dependent, rate of micronucleus increasing in Vicia faba root tip cell and mitotic index decreasing as well. It is feasible to monitor lead pollution using micronucleus test on root tip cell of Vicia faba.

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Available abstract

Objective To study the genetic effects of lead on root tip cell of Vicia faba. Methods Micronucleus tests were conducted on root tip cell of Vicia faba treated with Pb2+ of concentrations of 0.1, 1.0, 5.0, 25.0, 50.0 mg/L for 12-36 h. Results The results indicated that the rates of micronucleus of lead-treated tip cell were significantly higher than that of the control (P0.01),the mitotic index significantly decreased compared with the control (P0.05)and the response of high dose-short time exposure was the same as that of low dose-long time exposure. Conclusion Lead may induce, concentration-dependent and time-dependent, rate of micronucleus increasing in Vicia faba root tip cell and mitotic index decreasing as well. It is feasible to monitor lead pollution using micronucleus test on root tip cell of Vicia faba.

Key concepts: Vicia faba, Root tip, Micronucleus, Micronucleus test, Mitotic index, Mitosis, Negative control, Vicia

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