The research of human prostate cancer PC3 、LNCaP apoptosis induced by siRNA mediated survivin gene silencing

Objectives To study the influence of siRNA to the expression of survivin and the prostate cancer PC3 、LNCaP cell apoptosis.Methods Design 2 siRNA sequences aim at survivin genes,using siRNA design software; PC3,LNCaP cell culture,take siRNA into PC3 、LNCaP cells,through the liposome; MTT method to detect the proliferation of cells inhibition rate (IR) ; Flow cytometry to detect cell apoptosis; Half quantitative RT-PCR detection survivin mRNA expression level ; Western Blotting detection survivin protein expression strength.Results siRNA 2 groups of PC3 cells of proliferation inhibition rate (IR) after transfection:43.84％,54.31％ ; Apoptosis index (AI):22.3％,30.34％; Significantly higher than normal control group PC3 cells of proliferation inhibition rate (IR):2.40％ and apoptosis index (AI):3.81％ ; LNCaP cell proliferation of inhibition rate (IR):41.78％,53.31％ ; Apoptosis index (AI):18.33％,28.44％ ;Significantly higher than normal control group LNCaP cell proliferation of inhibition rate (IR):1.97％ and apoptosis index (AI):3.43％.Conclusions siRNA can inhibits the expression of survivin in PC3 、LNCaP cells,induce cell apoptosis,provide the trial basis for prostate cancer,especially metastatic castration-resistant prostate cancer. Key words: Prostatic Neoplasms;  Apoptosis