Study of the effect of siRNA targeted against survivin on cell proliferation and apoptosis of esophageal cancer cells

Objective To investigate the effect of siRNA targeted against survivin on cell proliferation and apoptosis of Eca-109 esophageal cancer cells. Methods A siRNA plasmid expression vector against survivin was constructed and transfected into Eca-109 cells with LipofectamineTM2000.The changes of survivin mRNA and protein expression were detected by semi-quantitive RT-PCR and Western Blot.Cell proliferation was detected by MTT assay.Cell apoptosis was evaluated by flow cytometry. Results The sequence-specific siRNA effectively suppressed survivin expressing at both mRNA and protein levels.Survivin expression surpression significantly inhibited the proliferation(at 24 h and 48 h after cell seeding,the proliferation inhibition rate was 21.05% and 33.96% respectively) and induced apoptosis of Eca-109 cells(at 24 h and 48 h after transfection,apoptosis rate was(8.03±1.05)% and(6.22±1.06)% respectively. Conclusions siRNA targeted against survivin can effectively suppress Eca-109 cell proliferation and significantly induce Eca-109 cell apoptosis.