siRNA Against Survivin Suppresses the Proliferation of Pancreatic Cancer Cell PC-2 and Induces Its Apoptosis

Objective Blocking the expression of Survivin with s1RNA, and the effects of suppressing the proliferation of PC-2 cell and inducing its apoptosis were investigated. Methods Constructed the s1RNA against Survivin plasmid expression vector and transfected it into PC-2 cell with lipofectamine(superscript TM) 2000, the changes of Survivin expression were detected by semi-quantitive RT-PCR and immunohistochemical method, The effect of suppressing the proliferation of PC-2 cell was detected by the method of MTT; the effect of inducing PC-2 cell apoptosis was detected by flow cytometry. Results The sequence specific siRNA can effectively block the expression of Survivin both at the mRNA and protein levels, the expression inhibition ratio was 81.25% at mRNA level and 74.24% at protein level; blocking the expression of Survivin can suppress the proliferation of PC-2 cell significantly, 24, 48 hours after the cell was reseeded, the proliferation inhibition ratio was 28.00% and 33.38% respectively; 24, 48 hours after the transfection, 8.46% and 7.53% cells were induced to apoptosis respectively. Conclusion The siRNA against Survivin plasmid expression vector constructed in the study can blocking the expression of Survivin in PC-2 cell effectively and specifically; blocking the expression of Survivin can significantly suppress the proliferation of PC-2 cell and induce a certain degree cells apoptosis; RNAi against Survivin is of a certain value in the gene therapy of pancreatic cancer.