2016•FigshareOpen access
Additional file 1: Figure S1. of miR-138-5p contributes to cell proliferation and invasion by targeting Survivin in bladder cancer cells
Rong Yang, Minghui Liu, Hongwei Liang, Suhan Guo, Xu Guo, Min Yuan, Huibo Lian, Xiang Yan, Shiwei Zhang, Xi Chen, Feng Fang, Hongqian Guo, Chen‐Yu Zhang
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Abstract
The role of siRNAs targeting Survivin and a Survivin overexpression plasmid in regulating the proliferative and invasive activities of bladder cancer cells. (A) Western blotting analysis to detect Survivin protein levels in T24 cells transfected with siRNAs targeting Survivin. (B) Western blotting analysis to detect Survivin protein levels in T24 cells transfected with the Survivin overexpression plasmid. (C) The CCK-8 viability assay was performed 12, 24, 36, and 48 hours after the transfection of T24 cells with si-Survivin#1. (D) The CCK-8 viability assay was performed 12, 24, 36, and 48 hours after the transfection of T24 cells with the Survivin overexpression plasmid. (E) and (F) Transwell analysis of invading T24 cells treated with si-Survivin#1. E: representative image; F: quantitative analysis. (G) and (H) Transwell analysis of invading T24 cells treated with the Survivin overexpression plasmid. G: representative image; H: quantitative analysis. *** p